Which molecules recognize specific codons and transport amino acids?
Which molecules recognize specific codons and transport amino acids?
Small RNA molecules known as transfer RNA (tRNA) recognize the individual codons on mRNA and carry the corresponding amino acids.
What are the differences between replication transcription and translation?
Differences. DNA replication occurs in preparation for cell division, while transcription happens in preparation for protein translation. DNA replication is important for properly regulating the growth and division of cells.
What direction is DNA read during replication?
5′ to 3′ direction
DNA replication is semiconservative. Each strand in the double helix acts as a template for synthesis of a new, complementary strand. New DNA is made by enzymes called DNA polymerases, which require a template and a primer (starter) and synthesize DNA in the 5′ to 3′ direction.
Why methionine is the start codon?
Because the first tRNA to bind to the peptidyl binding site (P site) in the initiation complex is always the initiator tRNA, tRNAfMET. tRNAfMET binds to the start codon of mRNA, AUG. The first amino acid of the protein is thus methionine.
What is analytical ultracentrifugation?
Analytical ultracentrifugation (AUC) is a powerful technique to determine the global conformational changes in RNA molecules mediated by cations or small molecule ligands.
What are the latest developments in ultracentrifugation?
Recent developments within the field of ultracentrifugation include air-cooled, benchtop centrifuges and a novel analytical centrifuge. The benchtop centrifuges are ideal for rapid, preparative work in small volumes and have also been used for analytical studies.
Can ultracentrifugation be used to study protein sedimentation?
Abstract Analytical ultracentrifugation (AU) is reemerging as a versatile tool for the study of proteins. Monitoring the sedimentation of macromolecules in the centrifugal field allows their hydrodynamic and thermodynamic characterization in solution, without interaction with any matrix or surface.
Can ultracentrifugation be used to recover OMVs from bacterial cultures?
Typically, bacterial cells are removed from the liquid culture by centrifugation and filtration, after which, OMVs are recovered from the clear liquid by ultracentrifugation. However, when industrial scale recovery of OMVs is required, ultracentrifugation might not be the most suitable method, so alternative approaches are required.